孟繁泽,曹锐,胡冰,秦磊,李春.细胞工厂设计数字化赋能绿色生物制造[J].中国科学院院刊,2025,40(1):67-78.

细胞工厂设计数字化赋能绿色生物制造

Digitalized design of cell factory enables green biomanufacturing

孟繁泽^1,2,3
清华大学 化学工程系 北京 100084;清华大学 工业生物催化教育部重点实验室 北京 100084;清华大学 合成与系统生物学中心 北京 100084
MENG Fanze^1,2,3
Department of Chemical Engineering, Tsinghua University, Beijing 100084, China;Key Laboratory for Industrial Biocatalysis, Ministry of Education, Tsinghua University, Beijing 100084, China;Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China
曹锐⁴
新疆大学 智能科学与技术学院(未来技术学院)乌鲁木齐 830049
CAO Rui⁴
School of Intelligence Science and Technology (School of Future Technology), Urumqi 830049, China
胡冰⁵
北京理工大学 化学与化工学院生物化工研究所/医药分子科学与制剂工程工信部重点实验室 北京 100081
HU Bing⁵
Institute of Biochemistry and Chemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology/Key Laboratory of Medicinal Molecular Science and Pharmaceutical Engineering, Beijing 100081, China
秦磊^1,2,3
清华大学 化学工程系 北京 100084;清华大学 工业生物催化教育部重点实验室 北京 100084;清华大学 合成与系统生物学中心 北京 100084
QIN Lei^1,2,3
Department of Chemical Engineering, Tsinghua University, Beijing 100084, China;Key Laboratory for Industrial Biocatalysis, Ministry of Education, Tsinghua University, Beijing 100084, China;Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China
李春^1,2,3,5*
清华大学 化学工程系 北京 100084;清华大学 工业生物催化教育部重点实验室 北京 100084;清华大学 合成与系统生物学中心 北京 100084;北京理工大学 化学与化工学院生物化工研究所/医药分子科学与制剂工程工信部重点实验室 北京 100081
LI Chun^1,2,3,5*
Department of Chemical Engineering, Tsinghua University, Beijing 100084, China;Key Laboratory for Industrial Biocatalysis, Ministry of Education, Tsinghua University, Beijing 100084, China;Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China;Institute of Biochemistry and Chemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology/Key Laboratory of Medicinal Molecular Science and Pharmaceutical Engineering, Beijing 100081, China

         新质生产力;绿色生物制造;细胞工厂;数字化;合成生物学

        new productive force;green biomanufacturing;cell factory;digitalization;synthetic biology

        绿色制造是一种综合考虑环境影响与资源效益的现代化制造模式，而绿色生物制造是绿色制造的重要方式之一，是新质生产力的重要组成部分。绿色生物制造以细胞工厂为核心，利用可再生资源，实现化学品的低碳、绿色、可持续的高效生产。其中，高性能细胞工厂的设计是实现高效绿色生物制造的重中之重。传统细胞工厂设计过程复杂，耗时耗力。随着人工智能领域的发展，数字化辅助细胞工厂设计节约了时间和成本。通过数据库构建与更新、代谢流调控数字化设计、目标分子逆合成途径数字化设计、基因线路数字化设计、发酵工艺数字孪生等技术的组合可实现全生命周期数字化细胞工厂的设计，指导高性能细胞工厂的快速构建与优化，赋能绿色生物制造。

        Green manufacturing is a modern manufacturing mode in the consideration of both environmental impact and efficiency of resource utilization, it represents a significant component of the “new productive force”. Centered on cell factories, green biomanufacturing leverages renewable resources to efficiently produce products that conform to the concepts of low-carbon, green, and sustainable development. The design of high-performance cell factories is pivotal for achieving efficient green biomanufacturing. Nevertheless, traditional cell factory design processes are complex, time-consuming, and labor-intensive. With advancements in artificial intelligence, digital-assisted cell factory design has emerged as a solution to save time and reduce costs. By integrating technologies such as database construction and updating, digital metabolic flux control design, digital retrosynthetic pathway design for target molecules, digital genetic circuit design, and fermentation process digital twin modeling, the full lifecycle digital design of cell factories can be achieved. This approach enables the rapid construction of high-performance cell factories, empowering green biomanufacturing.