崔颖璐,吴边.符合工程化需求的生物元件设计[J].中国科学院院刊,2018,33(11):1150-1157.
符合工程化需求的生物元件设计
Biological Components Design for Engineering Requirements
符合工程化需求的生物元件设计
Biological Components Design for Engineering Requirements
作者
崔颖璐
中国科学院微生物研究所 微生物资源前期开发国家重点实验室 生理与代谢工程重点实验室 北京 100101
CUI Yinglu
CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
吴边
中国科学院微生物研究所 微生物资源前期开发国家重点实验室 生理与代谢工程重点实验室 北京 100101
WU Bian
CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
中国科学院微生物研究所 微生物资源前期开发国家重点实验室 生理与代谢工程重点实验室 北京 100101
CUI Yinglu
CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
吴边
中国科学院微生物研究所 微生物资源前期开发国家重点实验室 生理与代谢工程重点实验室 北京 100101
WU Bian
CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
中文关键词
酶分子计算设计;酶工程;人工金属酶;底物选择性;热稳定性
英文关键词
computational enzyme design;protein engineering;artificial metalloenzymes;substrate selectivity;thermosability
中文摘要
从合成生物学的角度来认知生命，其本质是可数据化与可设计性。生命体中绝大多数的催化功能是由酶来实现的，因此催化元件是合成生物学中最核心的元件之一。序列决定构象，而构象则决定功能。基于空间结构的催化元件序列数字化设计，是合成生物学研究的重要热点和前沿领域。它既可为开发合成生物学功能器件，特别是全新化学催化器件提供大量原型分子，同时也为发展模块化、工程化调控元件提供设计模板和指导规律。文章针对近年来出现的生物元件，尤其是催化元件的前沿进展进行简要介绍。
英文摘要
Understanding life from the perspective of synthetic biology centers on the design, construction, and characterization of novel biological systems under the engineering design principles. Most catalytic functions in living organisms are performed by enzymes, which serve as one of the most important components in synthetic biology. While the amino acid sequence makes up the primary structure of the protein, the three-dimensional structure of protein determines its biochemical properties. Therefore, researches on biological functionality based on protein structure have become frontiers of the emerging field of synthetic biology. Meanwhile, the development of computational enzyme design algorithms can provide large amounts of prototype molecules for the synthetic biological devices, especially for new chemical catalytic devices, and provide design templates and guidelines for important components in synthetic biology. This study provides a brief overview of new advances in the construction and design of biological components, especially synthetic biological devices in recent years.
DOI10.16418/j.issn.1000-3045.2018.11.002
作者简介
崔颖璐 中国科学院微生物研究所助理研究员,博士。2015年毕业于吉林大学,曾获得"博士研究生国家奖学金""唐敖庆化学奖学金"等荣誉。主要研究方向为新型工业酶的计算改造设计。发表SCI期刊论文20余篇,主持国家自然科学基金青年基金项目以及中国博士后科学基金面上项目。
E-mail:cuiyinglu@im.ac.cn
CUI Yinglu Received the Ph.D.degree from Jilin University in 2015,and now serves as an Assistant Professor at Institute of Microbiology,Chinese Academy of Sciences.She focuses on the application of computational approaches to investigate the structures,functions,and dynamics of proteins.Her current research is directed towards the computational enzyme design.She has authored more than 20 journal articles and received honors such as National Scholarship for Ph.D.students and Tang Aoqing Chemistry Scholarship in recent years.Her research on computational enzyme design has been supported by the National Natural Science Foundation of China (NSFC) and China Postdoctoral Science Foundation.
E-mail:cuiyinglu@im.ac.cn
吴边 中国科学院微生物研究所"百人计划"研究员,博士生导师,微生物资源前期开发国家重点实验室副主任,获国家自然科学基金委"优青"项目资助。担任中国生物发酵产业协会理事,中国微生物学会酶工程专业委员会委员。致力于新型工业酶的发掘、工程改造设计与催化应用,近年来主要从事微生物酶计算机设计方面的工作。在Nature Chemical Biology、Angewandte Chemie International Edition、Trends in Biotechnology、ACS Catalysis等刊物发表数十篇论文。长期与工业界密切合作,曾参与德国巴斯夫(BASF)、荷兰皇家帝斯曼集团(DSM)等跨国化工企业的工业酶项目开发,与国内多家企业开展了联合研发工作,目前已经有多项技术实现商业转化与产业应用。
E-mail:wub@im.ac.cn
WU Bian A Principle Investigator at Institute of Microbiology,Chinese Academy of Sciences (CAS),obtained his Ph.D.from University of Groningen in 2010.He joined the Institute of Microbiology,CAS as a Principal Investigator in 2015.His research interests include computational enzyme design,industrial bioprocess development and enzymology.He published dozens of papers in many periodicals including Nature Chemical Biology,Angewandte Chemie International Edition,Trends in Biotechnology,ACS Catalysis.He has been working closely with industry for a long time and has participated in the development projects of industrial enzyme of multinational chemical enterprises such as BASF in Germany and DSM in the Netherlands.He has also carried out joint research with many domestic enterprises on the new technologies,many of which have been applied for commercial purposes and industrial development.
E-mail:wub@im.ac.cn
E-mail:cuiyinglu@im.ac.cn
CUI Yinglu Received the Ph.D.degree from Jilin University in 2015,and now serves as an Assistant Professor at Institute of Microbiology,Chinese Academy of Sciences.She focuses on the application of computational approaches to investigate the structures,functions,and dynamics of proteins.Her current research is directed towards the computational enzyme design.She has authored more than 20 journal articles and received honors such as National Scholarship for Ph.D.students and Tang Aoqing Chemistry Scholarship in recent years.Her research on computational enzyme design has been supported by the National Natural Science Foundation of China (NSFC) and China Postdoctoral Science Foundation.
E-mail:cuiyinglu@im.ac.cn
吴边 中国科学院微生物研究所"百人计划"研究员,博士生导师,微生物资源前期开发国家重点实验室副主任,获国家自然科学基金委"优青"项目资助。担任中国生物发酵产业协会理事,中国微生物学会酶工程专业委员会委员。致力于新型工业酶的发掘、工程改造设计与催化应用,近年来主要从事微生物酶计算机设计方面的工作。在Nature Chemical Biology、Angewandte Chemie International Edition、Trends in Biotechnology、ACS Catalysis等刊物发表数十篇论文。长期与工业界密切合作,曾参与德国巴斯夫(BASF)、荷兰皇家帝斯曼集团(DSM)等跨国化工企业的工业酶项目开发,与国内多家企业开展了联合研发工作,目前已经有多项技术实现商业转化与产业应用。
E-mail:wub@im.ac.cn
WU Bian A Principle Investigator at Institute of Microbiology,Chinese Academy of Sciences (CAS),obtained his Ph.D.from University of Groningen in 2010.He joined the Institute of Microbiology,CAS as a Principal Investigator in 2015.His research interests include computational enzyme design,industrial bioprocess development and enzymology.He published dozens of papers in many periodicals including Nature Chemical Biology,Angewandte Chemie International Edition,Trends in Biotechnology,ACS Catalysis.He has been working closely with industry for a long time and has participated in the development projects of industrial enzyme of multinational chemical enterprises such as BASF in Germany and DSM in the Netherlands.He has also carried out joint research with many domestic enterprises on the new technologies,many of which have been applied for commercial purposes and industrial development.
E-mail:wub@im.ac.cn
