王殿冰,崔宗强,张先恩.分子生物传感器与细胞内分子影像[J].中国科学院院刊,2017,32(12):1303-1312.
分子生物传感器与细胞内分子影像
Molecular Biosensors and Molecular Imaging in Cells
分子生物传感器与细胞内分子影像
Molecular Biosensors and Molecular Imaging in Cells
作者
王殿冰
中国科学院生物物理研究所 生物大分子国家重点实验室 北京 100101
Wang Dianbing
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
崔宗强
中国科学院武汉病毒研究所 病毒学国家重点实验室 武汉 430071
Cui Zongqiang
State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
张先恩
中国科学院生物物理研究所 生物大分子国家重点实验室 北京 100101
Zhang Xian-En
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
中国科学院生物物理研究所 生物大分子国家重点实验室 北京 100101
Wang Dianbing
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
崔宗强
中国科学院武汉病毒研究所 病毒学国家重点实验室 武汉 430071
Cui Zongqiang
State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
张先恩
中国科学院生物物理研究所 生物大分子国家重点实验室 北京 100101
Zhang Xian-En
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
中文关键词
分子生物传感器;细胞影像;分子信标;荧光共振能量转移;生物发光共振能量转移;双分子荧光互补
英文关键词
molecular biosensor;cell imaging;molecular beacon (MB);fluorescence resonance energy transfer (FRET);bioluminescence resonance energy transfer (BRET);bimolecular fluorescence complementation
中文摘要
分子生物传感器是由生物大分子通过基因重组或DNA合成所构成的传感器，能够实时、可视化探测活细胞及活体内关键分子事件。目前研究热度高、应用广的分子生物传感器包括分子信标（MB）、共振能量转移系统（荧光共振能量转移和生物发光共振能量转移）和分子荧光互补系统（如双分子荧光互补、三分子荧光互补等）。文章介绍了这几类分子生物传感器的原理和特点，重点强调了它们在活细胞分子影像学中的运用，如：研究细胞内蛋白之间的相互作用，探索生物大分子在细胞中的定位、运动和动力学等。此外，还讨论了分子生物传感器的局限性和面临的挑战，并展望了未来发展方向。"眼见为实"，分子生物传感器在这方面发挥独特的作用，它使我们前所未有地深入到细胞内部去观察生物分子事件乃至生物学过程，从而解答更多的生物学难题。
英文摘要
Molecular sensors are the biosensors made of macrobiomolecules. They are significant tools for real-time imaging of the molecular events in living cells and in vivo. Molecular beacons (MB), fluorescence/bioluminescence resonance energy transfer sensors (FRET/BRET) and molecular fluorescence complementation systems (BiFC, TriFC, etc.) are major types of molecular biosensors for live cell imaging applications. In this paper, the principles and characteristics of the molecular biosensors are described. The biosensors show their unique role in study of biomolecular interactions, localization, movement, and kinetics in living cells. The restrains, challenges, and future prospects of the molecular biosensors are also discussed. Because "Seeing is believing", the molecule biosensors enable us to see the biological process with super high spatio-temporal resolution, and thus to answer the fundamental biological questions.
DOI10.16418/j.issn.1000-3045.2017.12.004
作者简介
王殿冰 博士,中科院生物物理所副研究员,长期从事生物传感和分析病原微生物相关研究。发表SCI收录论文20余篇,拥有7项中国专利。主持和参与多项国家自然科学基金、"863"计划和中科院项目,曾获得湖北省自然科学奖一等奖。E-mail:wangdb@moon.ibp.ac.cn
Wang Dianbing Ph.D.,associated professor in Institute of Biophysics,Chinese Academy of Sciences (CAS).Her research interest focuses on biosensors and analytical pathogen microbiology.She has 7 patents and has published more than 20 papers in SCI indexed journals.She participated in a lot of important projects from National Natural Science Foundation of China,CAS,and was also awarded the First Prize of Natural Sciences in Hubei Province,China.E-mail:wangdb@moon.ibp.ac.cn
张先恩 男,中科院生物物理所研究员。1993年在中科院武汉病毒研究所晋升研究员,从事生物传感器、纳米生物学和分析微生物研究,发表论文240篇(SCI收录200篇),出版生物传感和生物芯片专著3本。曾在科技部基础研究司从事基础研究宏观管理。目前兼任中国生物工程学会副理事长,亚洲生物技术协会(AFOB)顾问和纳米生物技术、生物传感与生物芯片分会共同主席、APEC首席科学顾问会议中国代表(2013,2015和2016年)、国家"973"计划专家顾问组副组长。2015年被加拿大Alberta大学授予名誉科学博士学位。E-mail:zhangxe@ibp.ac.cn
Zhang Xian-En Male,distinguished professor in the Institute of Biophysics,Chinese Academy of Sciences (CAS).He became a full professor in the Institute of Virology,CAS,in 1993,specializing in biosensors,nanobiology,and analytical microbiology,and has published about 240 peer-reviewed papers and three books.He currently serves as the vice president of the Chinese Society of Biotechnology,co-chair of the Division of Nanobiotechnology,Biosensors and Biochips of the Asian Federation of Biotechnology (AFOB),executive chief editor of Chinese Journal of Biotechnology,editorial member or advisor for 6 other scientific journals.From 2002 to 2013,Dr.Zhang served as the director general of the Basic Research Department,Ministry of Science and Technology (MOST),dedicating himself to the macro management of science development in China.He has been serving as the Chinese representative of the APEC Meeting of Chief Science Advisors or Equivalents since 2013(except for 2014) and deputy head of the Expert Advisory Group of the National "973" Program.In 2015,he was awarded an Honorary Doctor of Science Degree by the University of Alberta,Canada.E-mail:zhangxe@ibp.ac.cn
Wang Dianbing Ph.D.,associated professor in Institute of Biophysics,Chinese Academy of Sciences (CAS).Her research interest focuses on biosensors and analytical pathogen microbiology.She has 7 patents and has published more than 20 papers in SCI indexed journals.She participated in a lot of important projects from National Natural Science Foundation of China,CAS,and was also awarded the First Prize of Natural Sciences in Hubei Province,China.E-mail:wangdb@moon.ibp.ac.cn
张先恩 男,中科院生物物理所研究员。1993年在中科院武汉病毒研究所晋升研究员,从事生物传感器、纳米生物学和分析微生物研究,发表论文240篇(SCI收录200篇),出版生物传感和生物芯片专著3本。曾在科技部基础研究司从事基础研究宏观管理。目前兼任中国生物工程学会副理事长,亚洲生物技术协会(AFOB)顾问和纳米生物技术、生物传感与生物芯片分会共同主席、APEC首席科学顾问会议中国代表(2013,2015和2016年)、国家"973"计划专家顾问组副组长。2015年被加拿大Alberta大学授予名誉科学博士学位。E-mail:zhangxe@ibp.ac.cn
Zhang Xian-En Male,distinguished professor in the Institute of Biophysics,Chinese Academy of Sciences (CAS).He became a full professor in the Institute of Virology,CAS,in 1993,specializing in biosensors,nanobiology,and analytical microbiology,and has published about 240 peer-reviewed papers and three books.He currently serves as the vice president of the Chinese Society of Biotechnology,co-chair of the Division of Nanobiotechnology,Biosensors and Biochips of the Asian Federation of Biotechnology (AFOB),executive chief editor of Chinese Journal of Biotechnology,editorial member or advisor for 6 other scientific journals.From 2002 to 2013,Dr.Zhang served as the director general of the Basic Research Department,Ministry of Science and Technology (MOST),dedicating himself to the macro management of science development in China.He has been serving as the Chinese representative of the APEC Meeting of Chief Science Advisors or Equivalents since 2013(except for 2014) and deputy head of the Expert Advisory Group of the National "973" Program.In 2015,he was awarded an Honorary Doctor of Science Degree by the University of Alberta,Canada.E-mail:zhangxe@ibp.ac.cn
