毛颖波,陈殿阳,陈晓亚.植物非编码RNA与抗虫防御反应的研究及应用[J].中国科学院院刊,2017,32(8):814-821.
植物非编码RNA与抗虫防御反应的研究及应用
Advances in Research of Plant Non-coding RNAs and RNAi-based Technology Against Insects Herbivore
植物非编码RNA与抗虫防御反应的研究及应用
Advances in Research of Plant Non-coding RNAs and RNAi-based Technology Against Insects Herbivore
作者
毛颖波
中国科学院上海生命科学研究院 植物生理生态研究所植物分子遗传国家重点实验室 上海 200032
Mao Ying-Bo
National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China
陈殿阳
中国科学院上海生命科学研究院 植物生理生态研究所植物分子遗传国家重点实验室 上海 200032;中国科学院大学 北京 100049
Chen Dian-Yang
National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China;University of Chinese Academy of Sciences, Beijing 100049, China
陈晓亚
中国科学院上海生命科学研究院 植物生理生态研究所植物分子遗传国家重点实验室 上海 200032
Chen Xiao-Ya
National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China
中国科学院上海生命科学研究院 植物生理生态研究所植物分子遗传国家重点实验室 上海 200032
Mao Ying-Bo
National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China
陈殿阳
中国科学院上海生命科学研究院 植物生理生态研究所植物分子遗传国家重点实验室 上海 200032;中国科学院大学 北京 100049
Chen Dian-Yang
National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China;University of Chinese Academy of Sciences, Beijing 100049, China
陈晓亚
中国科学院上海生命科学研究院 植物生理生态研究所植物分子遗传国家重点实验室 上海 200032
Chen Xiao-Ya
National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China
中文关键词
非编码RNA;微RNA (miRNA);RNA干扰(RNAi);抗虫
英文关键词
non-coding RNA;microRNA;RNA interference;insect resistance
中文摘要
非编码RNA参与了多种重要的生命进程，是当今研究的热点领域。在植物中，非编码RNA除了参与维持基因组的稳定，还在生长、发育、逆境胁迫反应等过程中发挥重要作用。RNA干扰（RNA interference，RNAi）是指由RNA触发的相应基因的表达抑制，是非编码RNA调控基因表达的一种重要的作用方式，自发现以来已逐渐发展为遗传分析、疾病治疗以及植物保护等方面的有效的新技术。文章重点介绍了微RNA（microRNA，miRNA）在植物抗虫防御中的生物学功能，siRNA对植物防御记忆的影响以及RNAi对植物防御信号途径的调节作用，同时阐述了RNAi在提高农作物抗虫性上的应用并展望了未来植物非编码RNA的研究方向。
英文摘要
Non-coding RNAs (ncRNAs), including long non-coding RNAs (lncRNAs) and small RNAs such as microRNAs (miRNAs) and short interference RNAs (siRNAs), exist widely in eukaryotes and affect gene expressions at different levels. In plant, ncRNAs are involved in growth, development, response to biotic and abiotic stresses, and are important players in epigenetics. RNA interference (RNAi) refers to homology gene silencing caused by double strand RNAs (dsRNAs) and is a major mechanism of gene expression regulation by ncRNAs. Since discovery, RNAi has been developed as a powerful technology for genetic analysis, gene therapy, and plant protection. In this review, we summarize the recent progresses in research of the function and mechanism of ncRNAs in plant defense pathways and discuss the application of RNAi technology in agricultural pest control.
作者简介
毛颖波 中科院上海生命科学院植物生理生态所研究员。研究方向为植物与昆虫的防御互作,主要工作包括:棉铃虫对棉酚的耐受性机制,次生代谢物与植物-昆虫互作/共进化,植物抗虫反应中的非编码RNA,植物介导的昆虫RNAi新技术,昆虫效应子作用机制等。获2010年全国优秀博士学位论文奖。主持和承担国家基金委、重大专项、重点专项和中科院B类先导课题等多个项目的研究。E-mail:ybmao@sibs.ac.cn
Mao Ying-Bo Received B.Sc.from School of Life Sciences,Xiamen University in 1997 and Ph.D.from the Shanghai Institute of Plant Physiology and Ecology,Chinese Academy of Sciences (CAS).Her Ph.D.dissertation was rated as excellent doctoral dissertation of CAS (2009) and national excellent doctorial dissertation (2010).She has worked in the Shanghai Institute of Plant Physiology and Ecology.Dr Mao's main interest is in studying the defense pathways underlying insect-plant interactions,including the mechanism of gossypol tolerance in bollworm,plant mediated RNA interference in insects,and molecular mechanism of insect effectors interfere with plant defense.E-mail:ybmao@sibs.ac.cn
陈晓亚 男,中科院院士,发展中国家科学院院士,中科院上海生科院植物生理生态所研究员、上海辰山植物科学中心主任。从事植物次生代谢、RNA干扰抗虫新技术、棉纤维发育等方面取得了成绩。对植物倍半萜,尤其是棉酚和青蒿素生物合成途径及调控开展了系统深入的研究,解析了植物激素和miRNA调控倍半萜生物合成的分子机制,发展了植物介导的RNA干扰抗虫技术,成为研发新一代植物抗虫技术的重要突破。阐述了植物激素(赤霉素)调控棉纤维伸长的机制,发现miRNA靶基因调控表皮毛发育,对揭示棉纤维和植物表皮毛细胞发育的分子机制具有重要意义。至今已发表论文百余篇,包括Nature Biotechnology,Nature Communications,Plant Cell等重要刊物。现任中国植物生理和分子生物学学会理事长、Science Bulletin主编、Plant Biotechnology Journal副主编等职。2008年获得何梁何利科学技术进步奖,2010年获全国优秀博士学位论文指导教师奖,2014年获第三届国际棉花基因组组织(ICGI)杰出贡献奖。E-mail:xychen@sibs.ac.cn
Chen Xiao-Ya Male,received B.Sc.from Nanjing University,China in 1982,and Ph.D.from Reading University,UK in 1985.Then he worked in Nanjing University (lecturer,associate professor);Institute of Botany,Tuebingen University,Germany (visiting scientist),and Department of Medicinal Chemistry and Pharmacognosy,Purdue University,USA (postdoctoral fellow).Since 1994,he has been a group leader in Shanghai Institute of Plant Physiology and Ecology,Chinese Academy of Sciences.He is interested in plant secondary metabolism,plantinsect interactions,new technology (RNAi) for pest control,trichome and cotton fiber development.He received HLHL Foundation Prize for Scientific and Technological Progress in 2008,and The Third ICGI Award for Outstanding Contribution to Cotton Genomics in 2014.Currently he also serves as President of Chinese Society for Plant Physiology and Molecular Biology,Director of Research Center of Shanghai Chenshan Botanical Garden,Editor-in-Chief of Science Bulletin,and Associate Editor of Plant Biotechnology Journal.He was elected a member (Academician) of Chinese Academy of Sciences in 2005 and a member of The World Academy of Sciences (TWAS) for the advancement of science in developing countries in 2008.E-mail:xychen@sibs.ac.cn
Mao Ying-Bo Received B.Sc.from School of Life Sciences,Xiamen University in 1997 and Ph.D.from the Shanghai Institute of Plant Physiology and Ecology,Chinese Academy of Sciences (CAS).Her Ph.D.dissertation was rated as excellent doctoral dissertation of CAS (2009) and national excellent doctorial dissertation (2010).She has worked in the Shanghai Institute of Plant Physiology and Ecology.Dr Mao's main interest is in studying the defense pathways underlying insect-plant interactions,including the mechanism of gossypol tolerance in bollworm,plant mediated RNA interference in insects,and molecular mechanism of insect effectors interfere with plant defense.E-mail:ybmao@sibs.ac.cn
陈晓亚 男,中科院院士,发展中国家科学院院士,中科院上海生科院植物生理生态所研究员、上海辰山植物科学中心主任。从事植物次生代谢、RNA干扰抗虫新技术、棉纤维发育等方面取得了成绩。对植物倍半萜,尤其是棉酚和青蒿素生物合成途径及调控开展了系统深入的研究,解析了植物激素和miRNA调控倍半萜生物合成的分子机制,发展了植物介导的RNA干扰抗虫技术,成为研发新一代植物抗虫技术的重要突破。阐述了植物激素(赤霉素)调控棉纤维伸长的机制,发现miRNA靶基因调控表皮毛发育,对揭示棉纤维和植物表皮毛细胞发育的分子机制具有重要意义。至今已发表论文百余篇,包括Nature Biotechnology,Nature Communications,Plant Cell等重要刊物。现任中国植物生理和分子生物学学会理事长、Science Bulletin主编、Plant Biotechnology Journal副主编等职。2008年获得何梁何利科学技术进步奖,2010年获全国优秀博士学位论文指导教师奖,2014年获第三届国际棉花基因组组织(ICGI)杰出贡献奖。E-mail:xychen@sibs.ac.cn
Chen Xiao-Ya Male,received B.Sc.from Nanjing University,China in 1982,and Ph.D.from Reading University,UK in 1985.Then he worked in Nanjing University (lecturer,associate professor);Institute of Botany,Tuebingen University,Germany (visiting scientist),and Department of Medicinal Chemistry and Pharmacognosy,Purdue University,USA (postdoctoral fellow).Since 1994,he has been a group leader in Shanghai Institute of Plant Physiology and Ecology,Chinese Academy of Sciences.He is interested in plant secondary metabolism,plantinsect interactions,new technology (RNAi) for pest control,trichome and cotton fiber development.He received HLHL Foundation Prize for Scientific and Technological Progress in 2008,and The Third ICGI Award for Outstanding Contribution to Cotton Genomics in 2014.Currently he also serves as President of Chinese Society for Plant Physiology and Molecular Biology,Director of Research Center of Shanghai Chenshan Botanical Garden,Editor-in-Chief of Science Bulletin,and Associate Editor of Plant Biotechnology Journal.He was elected a member (Academician) of Chinese Academy of Sciences in 2005 and a member of The World Academy of Sciences (TWAS) for the advancement of science in developing countries in 2008.E-mail:xychen@sibs.ac.cn
